Using Peroxidase-like Phosphate Hydrate Nanosheets for Highly Sensitive Dopamine Detection

Date:Mar 21, 2023    |  【 A  A  A 】

Marine microorganisms are considered as one of the most active parts in marine ecosystems. Utilizing marine microbial resources became a key point in marine biotechnology. Especially, the biosynthesis of functional nanoparticles with the regulation of marine microorganisms have attracted much attention.

Recently, the research team led by Assoc. Prof. ZHAI Xiaofan from the Institute of Oceanology of the Chinese Academy of Sciences (IOCAS), cooperating with Prof. TANG Xuexi from Ocean University of China (OUC) firstly reported a colorimetric sensor built by zinc phosphate hydrate nanosheets (SA@ZnPNS).

The study was published in Colloids and Surfaces B: Biointerfaces on Mar. 6.

In this study, researchers proposed a novel biosynthesis strategy of SA@ZnPNS using Shewanella algae, which was efficient, environmentally friendly, and low cost. SA@ZnPNS was characterized and optimized by two important parameters of pH value and precursors concentration. SA@ZnPNS showed an excellent peroxidase-like activity which can catalyze H2O2 to ·OH and then turn 3,3',5,5'-tetramethylbenzidine (TMB) to oxidation state, causing a color variation to blue.

Based on the properties of SA@ZnPNS, a fast colorimetric method for dopamine (DA) quantitative analysis was proposed. The detection mechanism was clarified as DA consumed quantified ·OH and caused a lightened blue color of reaction system. The linear range of DA detection was 0.1-40 μM, and detection limit was 0.083 μM. The colorimetric method showed a wide detection range, low detection limit, relatively good anti-interference, and outstanding performance in the detection of actual serum samples.

"Our research provided a novel, green, safe and sustainable strategy for the bio-nanoparticle applied in bio-sensing field," said YANG Jing, first author of the study.

"Novel nanoparticles biosynthesis strategy is an emerging, open area of research with many possibilities. Therefore, obtaining bio-nanoparticles with excellent properties and expanding applications in photovoltaic cells, hydrogen production, medicine, marine corrosion and protection, agriculture, and other fields deserve further investigation, which are also our future research directions," added Assoc. Prof. ZHAI.

This work was supported by Shandong Provincial Natural Science Foundation of China, the Young Elite Scientists Sponsorship Program by CAST, Wenhai Program of the S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao).

Schematic of DA detection by SA@ZnPNS

Jing Yang, Xiaofan Zhai*, Xucheng Dong, Liuhui Zhao, Yu Zhang, Hui Xiao*, Peng Ju, Jizhou Duan, Xuexi Tang, Baorong Hou. (2023). Peroxidase-like phosphate hydrate nanosheets bio-synthesized by a marine Shewanella algae strain for highly sensitive dopamine detection, Colloids and Surfaces B: Biointerfaces, 225: 113248.

(Text by ZHAI Xiaofan)

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(Editor: ZHANG Yiyi)

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