NInstitute of Oceanology,Chinese Academy of Sciences


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Name:(姓名):Chen Huaxin
Title:(职称):Associate Professor
Research Field:(研究领域):Algal Biology & Biotechnology

2012-present: Associate Professor, Institute of Oceanology, Chinese Academy of Sciences; Key Lab of Experimental Marine Biology
2007-2012: Assistant Professor, Institute of Oceanology, Chinese Academy of Sciences; Key Lab of Experimental Marine Biology
2004-2007: Postdoctoral Research Assistant, State Key Lab of Bioreactor Engineering, East China University of Science and Technology

Ph.D. Botany, Shandong Agricultural University, 1999-2004,
B.A. Agronomy, Yangtze University, 1995-1999

Professional Memberships:(组织任职)

Research Interests:(研究方向)
Algal genetics and metabolic engineering, Algae cultivation and wastewater treatment, Production of high value compounds

Honors and Awards:(获得荣誉、获奖情况)
First Class Award of Marine Science and Technology (2012)

Selected Publications:(发表论文、专利、出版书籍等):
1) Chen, H.*, Jiang, P. Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein. Microbial Cell Factoctories. 2019;18(1):58. doi: 10.1186/s12934-019-1100-6
2) Chen, H.*, Jiang, P. Combinational biosynthesis and characterization of fusion proteins with tandem repeats of allophycocyanin holo-α subunits, and their application as bright fluorescent labels for immunofluorescence assay. Journal of Bioscience and Bioengineering 2018, 126 (6), pp.778, DOI: 10.1016/j.jbiosc.2018.06.004
3) Zhao, J., Jiang, P., Qiu, R., Ma, Y., Wu, C., Fu, H., Chen, H., Li, F. The Yellow Sea green tide: A risk of macroalgae invasion. Harmful Algae, 2018, 77,pp.11, DOI: 10.1016/j.hal.2018.05.007
4) Wu, J., Chen, H.*, Jiang, P. Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin α subunit, Bioengineered, 2018,9(1), pp.108, DOI: 10.1080/21655979.2017.1321282
5) Zhu, X., Chen, H.,Qi, H.,Jiang, P., Li, F.,Li, R. Optimization of Expression of Recombinant Phycobiliproteins in Escherichia coli and Assessment of Their Spectral Properties and Antioxidant Activity. 2017, Modern Food Science and Technology 33 (7), pp.43, DOI: 10.13982/j.mfst.1673-9078.2017.7.007
6) Ge, B., Lin, X., Chen, Y.,Wang, X.,Chen, H.,Jiang, P.,Huang, F. Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay. Process Biochemistry, 2017, 58, pp.306, DOI: 10.1016/j.procbio.2017.05.006
7) Wu, J., Chen, H.*, Zhao, J., Jiang, P. Fusion proteins of streptavidin and allophycocyanin alpha subunit for immunofluorescence assay. Biochemical Engineering Journal. 2017,125, pp.97, DOI:
8) Chen, H.*, Liu, Q., Zhao, J., Jiang, P. Biosynthesis, spectral properties and thermostability of cyanobacterial allophycocyanin holo-α subunits. International Journal of Biological Macromolecules, 2016, 88, pp.88, DOI: 10.1016/j.ijbiomac.2016.03.050
9) Chen, H., Jiang, P., Li, F., Wu, H. Improving Production of Thermostable and Fluorescent Holo-β-Allophycocyanin by Metabolically Engineered Escherichia coli Using Response Surface Methodology. Preparative Biochemistry and Biotechnology, 2015, 45 (7), pp.730, DOI: 10.1080/10826068.2014.943374
10) Zhao, J., Jiang, P., Qin, S., Liu, X., Liu, Z., Lin, H., Li, F., Chen, H., Wu, C. Genetic analyses of floating Ulva prolifera in the Yellow Sea suggest a unique ecotype. Estuarine, Coastal and Shelf Science, 2015, 163, pp.96, DOI: 10.1016/j.ecss.2015.05.027
11) Zhang, H., Li, F., Chen, H., Zhao, J., Yan, J., Jiang, P., Li, R., Zhu, B. Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome. Chinese Journal of Oceanology and Limnology, 2015, 33 (4) ,pp.819, DOI: 10.1007/s00343-015-4170-2
12) Hu, X., Chu, J., Zhang, S., Zhuang, Y., Wu, X., Chen, H., Lv, Z., Yuan, Z. An alkaline pH control strategy for methionine adenosyltransferase production in Pichia pastoris fermentation. Biotechnology and Bioprocess Engineering, 2014, 19 (5) , pp.900, DOI: 10.1007/s12257-014-0046-0
13) Zhou, S., Chen, H., Jiang, P., Li, F., Tang, D. Antioxidant activity of recombinant allophycocyanin β-subunit (Holo-apcB), Modern Food Science and Technology, 2014, 30 (8), pp.112, EID: 2-s2.0-84907234308
14) Chen, H., Lin, H., Li, F., Jiang, P., Qin, S. Biosynthesis of a stable allophycocyanin beta subunit in metabolically engineered Escherichia coli. Journal of Bioscience and Bioengineering, 2013, 115 (5), pp.485, DOI: 10.1016/j.jbiosc.2012.11.008
15) Chen, H., Lin, H., Jiang, P., Li, F., Qin, S. Genetic transformation of marine cyanobacterium Synechococcus sp. CC9311 (Cyanophyceae) by electroporation. Chinese Journal of Oceanology and Limnology, 2013, 31 (2) ,pp.416, DOI: 10.1007/s00343-013-2164-5
16) Jiang, P., Chen, Y., Liu, S., Cui, Y., Chen, H., Li, F., Qin, S. Biosynthesis and immobilization of biofunctional allophycocyanin. Evidence-based Complementary and Alternative Medicine, 2011, DOI: 10.1155/2011/751452
17) Liu, S., Chen, Y., Lu, Y., Chen, H., Li, F., Qin, S. Biosynthesis of fluorescent cyanobacterial allophycocyanin trimer in Escherichia coli. Photosynthesis Research, 2010, 105 (2),pp.135, DOI: 10.1007/s11120-010-9574-4
18) Liu, S., Chen, H., Qin, S., Zhang, W., Guan, X., Lu, Y. Highly soluble and stable recombinant holo-phycocyanin alpha subunit expressed in Escherichia coli. Biochemical Engineering Journal, 2009, 48 (1), pp.58, DOI: 10.1016/j.bej.2009.08.006
19) Zhang, W., Guan, X., Yang, Y., Ge, B., Chen, H., Li, F., Qin, S. Biosynthesis of fluorescent allophycocyanin α-subunits by autocatalysis in Escherichia coli. Biotechnology and Applied Biochemistry, 2009, 52 (2) ,pp.135, DOI: 10.1042/ba20070187
20) Hu, X., Chu, J., Zhang, Z., Zhang, S., Zhuang, Y., Wang, Y., Guo, M., Chen, H.., Yuan, Z. Effects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by Pgap -driven Pichia pastoris overexpressing methionine adenosyltransferase. Journal of Biotechnology, 2008, 137 (1-4), pp.44, DOI: 10.1016/j.jbiotec.2008.04.009
21) Chen, H., Chu, J., Zhang, S., Zhuang, Y., Qian, J., Wang, Y., Hu, X. Intracellular expression of Vitreoscilla hemoglobin improves S-adenosylmethionine production in a recombinant Pichia pastoris. Applied Microbiology and Biotechnology, 2007, 74 (6) ,pp.1205, DOI: 10.1007/s00253-006-0705-y
22) Chen, H., Li, P., Gao, H. Alleviation of photoinhibition by calcium supplement in salt-treated Rumex leaves. Physiologia Plantarum, 2007, 129 (2),pp.386, DOI: 10.1111/j.1399-3054.2006.00830.x
23) Chen, H., Gao, H., An, S., Li, W. Dissipation of excess energy in Mehler-peroxidase reaction in Rumex leaves during salt shock. Photosynthetica, 2004, 42 (1) , pp.117, DOI: 10.1023/
24) Chen, H., An, S, Li, W, Gao, H, Zou, Q. Enhancement of the Mehler-peroxidase reaction in salt-stressed Rumex K-1 leaves. Acta Botanica Sinica, 2004, 46 (7) ,pp.811, EID: 2-s2.0-5044233532
25) Chen, H., An, S.Z., Li, W.J., Gao, H.Y. NaCl stress increases the heat tolerance of PSII in leaves of Rumex K-1 seedlings. Journal of plant physiology and molecular biology, 2004 30 (3), pp.345, EID: 2-s2.0-16544394343
26) Chen, H., Li, W., An, S., Gao, H. Characterization of PSII photochemistry and thermostability in salt-treated Rumex leaves. Journal of Plant Physiology, 2004, 161 (3), pp.257, DOI: 10.1078/0176-1617-01231

1) Chen H., P. Jiang, F. Li, H. Lin. A thermostable recombinant allophycocyanin β subunit mutants. 2013. Chinese National Invention Patent, Patent Number: CN102942620A
2) Chen H. Wu J, Jiang Peng, Zhao J. A method for preparation of fusion fluorescent proteins in E. coli. Chinese National Invention Patent, Patent No. ZL201510426302X
3) Chen Y, Qin S, Wu N, Cui Y, Jiang P, Chen H, Li F. Application of a streptavidin-binding functional fluorescent magnetic nanoparticle. Chinese National Invention Patent, Patent No. ZL201010622840.3
4) Liu S, Chen Y, Lu Y, Qin S, Chen H, Li F. A method for preparing recombinant allophycocyanin trimer fluorescent protein. Chinese National Invention Patent, Patent No. ZL200910255643.X
5) Chen Y, Qin S, Liu S, Cui Y, Jiang P, Chen H, Li F. A method for preparing streptavidin-binding functional fluorescent magnetic nan- particles. Chinese National Invention Patent, Patent No. ZL201010261766.7
6) Qin S, Chen H, Liu S, A method for preparing highly stable fluorescent fusion phycocyanin. Chinese National Invention Patent, Patent No. ZL200910019914.1

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